产过氧化氢酶菌株CE-1 的筛选与鉴定     

张增祥  王伟  郝建华  牛瑞  孙谧 《海洋科学》2010,34(11):54-58

经过含H2O2的平板初筛和摇瓶发酵复筛,从保存的水样中筛选到一株过氧化氢酶高产菌株CE-1,其所产过氧化氢酶活性和比活分别为1 356.2 U/mL和3 401 U/mg。菌株CE-1为革兰氏阴性杆状细菌,根据其形态特征、16S rRNA基因序列系统进化树分析和生理生化特性,以及属内不同种间性状差异等因素的综合分析,将菌株CE-1鉴定为气单胞属(Aeromonas)细菌。  相似文献   

2008年1次沙尘暴事件对厦门岛大气中SO2等污染物含量的影响     

汪建君  陈立奇  张远辉  杨绪林  孙霞  李伟  詹建琼  徐国杰 《台湾海峡》2010,29(3):297-303

2008年5月27～29日源自内蒙古南部的沙尘暴于5月30日到达厦门.受其影响,30日晚厦门风速最高达到12.6 m/s;31日本监测站大气中CO、NO2和NO含量明显下降,分别从551.3μg/m^3下降至218.9μg/m^3,从25.0μg/m^3下降至6.7μg/m^3,从8.1μg/m^3下降至0.1μg/m^3;6月1日其分别回升至682.4、28.7、6.5μg/m^3.与CO、NO2和NO不同,PM10和SO2含量在5月31日没有明显变化,PM10含量从5月31日晚的10.1μg/m^3上升至6月1日的232.6μg/m^3,SO2含量从5月底的25.0μg/m^3上升至52.0μg/m^3,并且两者的变化显示出较好的相关性（R2=0.65）.据此认为本次沙尘暴到达厦门时,在引起PM10含量增加的同时还引起了SO2含量的升高.这是沙尘暴引起中国南方城市SO2含量升高的首次报道.尽管目前SO2在中国的分布和传输已有较多研究,但对于沙尘暴在中国南方城市传输SO2的机制仍需进一步的探讨.  相似文献   

Modelling combined open channel flow by artificial neural networks     

Han‐Chung Yang  Fi‐John Chang 《水文研究》2005,19(18):3747-3762

Combined open channel flow is encountered in many hydraulic engineering structures and processes, such as irrigation ditches and wastewater treatment facilities. Extensive experimental studies have conducted to investigate combined flow characteristics. Nevertheless, there is no simple relationship that can fully describe the velocity profiles in a turbulent flow. The artificial neural network (ANN) has great computational capability for solving various complex problems, such as function approximation. The main objective of this study is to evaluate the applicability of the ANN for simulating velocity profiles, velocity contours and estimating the discharges accordingly. The velocity profiles measured by an acoustic doppler velocimeter in the open channel of the Chihtan purification plant, Taipei, with different discharges at fixed measuring section and different depths are presented. The total number of data sets is 640 and the data sets are split into two subsets, i.e. training and validation sets. The backpropagation algorithm is used to construct the neural network. The results demonstrate that the velocity profiles can be modelled by the ANN, and the ANN constructed can nicely fit the velocity profiles and can precisely predict the discharges for the conditions investigated. Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

Rate and historical change of direct recharge from precipitation constrained by unsaturated zone profiles of chloride and oxygen‐18 in dry river bed of North China Plain     

Ruiqiang Yuan  Xianfang Song  Dongmei Han  Yinghua Zhang  Liang Zhang  Bing Zhang  Xiting Long  Yilei Yu 《水文研究》2012,26(9):1291-1301

Quantifying of direct recharge derived from precipitation is crucial for assessing sustainability of well‐irrigated agriculture. In the North China Plain, the land use is dominated by groundwater‐irrigated farmland where the direct recharge derived from precipitation and irrigation. To characterize the mean rate and historical variance of direct recharge derived from precipitation, unsaturated zone profiles of chloride and δ¹⁸O in the dry river bed of the Beiyishui River were employed. The results show that archival time scale of the profile covers the duration from 1980 to 2002 (corresponding to depths from 5 to 2 m) which is indicated by matching the δ¹⁸O peaks in the isotope profile with the aridity indexes gained by instrumental records of annual precipitation and annual potential evaporation. Using the chloride mass balance method, the mean rate of the direct recharge corresponding to the archival time scale is estimated to be 3·8 ± 0·8 mm year^?1, which accounts for about 0·7% of the long‐term average annual precipitation. Further, the direct recharge rates vary from 2·1 to 6·8 mm year^?1 since 1980. Despite the subhumid climate, the estimate of recharge rates is in line with other findings in semiarid regions. The low rate of direct recharge is considered as a result of the relative dry climate in recent decades. In dry river bed, unsaturated zone profiles of chloride and δ¹⁸O combined with instrumental records could offer valuable information about the direct recharge derived from precipitation during droughts. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   

大黄鱼肌肉组织cDNA全长文库的构建及EST分析   总被引：1，自引：0，他引：1  

黄伟  薛良义  李婷  杨斌 《台湾海峡》2010,29(2):189-195

以大黄鱼肌肉组织为材料,利用Creator Smart cDNA Library Construction Kit构建了大黄鱼肌肉组织的全长cDNA文库.文库质量分析表明：cDNA文库容量不低于1.68×106cfu/mm3,重组率达96%,插入片断的平均长度大于1 000bp.随机挑取512个cDNA克隆进行5’端测序,其中430条ESTs的长度大于100bp;并初步拼接得到了230个单基因簇（unigene）,其中包括58个重叠群（contigs）,172个单拷贝ESTs（singletons）,冗余度为46.51%.经检索,35个ESTs在BLASTx上无明显的同源性（E值≤1.00×10-10）,为新基因.195个ESTs与已报道的基因有较高的同源性;其中与能量相关基因的ESTs丰度最高,占总数的20.00%.蛋白质合成、细胞骨架和信号传导次之,比例分别为13.48%、12.17%、10.00%,其中包括高迁移率族蛋白B1、瘦素受体、β1-热激蛋白等.这些EST数据为进一步筛选和克隆大黄鱼肌肉特异性表达基因提供了平台.  相似文献   

宽窄相间河道水流紊动特性试验研究   总被引：3，自引：0，他引：3       下载免费PDF全文

王文娥  廖伟  漆力健 《水科学进展》1990,31(3):394-403

为了探索宽窄相间河道的水流紊动特性，以西南地区宝兴河上游宽窄相间河段为研究对象，基于室内概化模型试验，采用多普勒声学流速仪（ADV）测量了室内模型典型断面上的三维瞬时流速，分析典型断面上的纵向时均流速、紊动强度、雷诺切应力和紊动能的分布规律。试验结果显示：宽窄相间水槽中，扩散段边壁的紊动强度大于中心区域的紊动强度，最大值位于0.2倍水深处；扩散段两侧坡脚处紊动能最大；侧壁区的平面和立面雷诺切应力最大值出现在扩散段内，中心区域最大雷诺切应力位于两槽间的中间断面处；扩散段内水流紊乱，两侧出现旋涡和涡脱，易造成侧壁侵蚀加强，引起河道拓宽。深入分析了宽窄相间河道水流的紊动特性，可为山区河流治理和自然灾害防治提供参考。  相似文献   

肇东和亳县陨石中的黑包体及其成因意义     

侯渭  王明再 《岩石学报》1988,4(3)

肇东、毫县陨石中的黑包体在总体成分、形状、大小上与陨石球粒相似,但两者的内部结构以及矿物组合不同。黑包体中矿物呈密堆状,主要由细粒橄榄石以及其它硅酸盐微晶组成,不含火成玻璃等特点表明黑包体未经历过熔融,它们可能是形成球粒的毛坯。因此认为球粒的形成有三个阶段:星云凝聚形成尘粒—尘粒吸积形成黑包体—黑包体熔融形成球粒。  相似文献   

冀中拗陷古潜山油田水化学研究   总被引：3，自引：0，他引：3       下载免费PDF全文

韩宝平 《地质科学》1989,(3):263-273

冀中拗陷油气田87%的储量产于海相碳酸盐岩古潜山中,在油气生成、运移、集聚和散失的过程中地下水起了重要作用。本文根据50个试样的水化学分析资料,运用对应分析方法对冀中拗陷古潜山油田水进行了分类并初步探讨了水化学成分演化与油气藏形成的关系。  相似文献   

Oceannographic Chemistry of Calcium Carbonate in the Sea Area at the Pearl River Mouth     

韩舞鹰  王均德 《中国地球化学学报》1989,8(2):179-186

Based on the analytical results for 101 samples from 50 survey spots,this paper describes the changes in rations of various components in calcium carbonate from the sea area at the Pearl River mouth with pH.Relations of ∑CO2 with salinity,and Ca concentration with salinity in different parts of the river mouth have been established.The annual throughput of Ca from the Pearl River and the saturation degrees of calcite and aragonite have been calculated.  相似文献   

肇东陨石高压加热—熔融实验研究   总被引：3，自引：4，他引：3  

侯渭  王道德 《岩石学报》1989,5(2):1-8

在30kbar压力下进行了肇东陨石的加热-熔融实验。实验结果表明。从840℃至2050℃,陨石中的金属-硫化物相经历了熔融—两相混溶的过程,最终形成一种Fe、Ni、Co、S之比不相等的多相混合物。而陨石中的硅酸盐相经历了热变质—熔融—熔融分异的过程。初熔温度为1610℃,2050℃时熔体发生分异,有富Si、Al的硅酸盐新相分离出来。以上述结果为依据,本文对类地行星早期层圈构造的形成机制进行了探讨和推论。  相似文献